Uni pfu

125 unit

10X PCR buffer

1 ml

EP-003A

250 unit

10X PCR buffer

1 ml

EP-003B

Uni pfu
(with dNTPs)

250 unit

10X PCR buffer

1 ml

EPD-003A

2.5 mM dNTPs

0.8 ml

500 unit

10X PCR buffer

1 ml

EPD-003B

 2.5 mM dNTPs

1.6 ml

Uni pfu ( 2.5 U/㎕)

1 ㎕

2.5 mM dNTPs

8 ㎕

10 X PCR buffer I, II or mix

10 ㎕

Template DNA*

< 1 ㎕

Primer 1 (10 pmole)

0.2 ~ 2 ㎕

Primer 2 (10 pmole)

0.2 ~ 2 ㎕

Sterilized deionized water

Up to 100 ㎕

*Template DNA
- Animal genomic DNA : 10 ~ 50 ng (35 ~ 40 cycle), 50 ~ 200 ng (25 ~ 35 cycle)
- Bacterial genomic DNA : 1 ~ 5 ng (30 ~ 35 cycle), 10 ~ 50 ng (20 ~ 25 cycle)
- Plasmid and lamda DNA : 1 ~ 5 ng (20 ~ 30 cycle)

Cation : Uni pfu DNA polymerase must be added after adding dNTP mixture into a tube. When there is no dNTP mixture in a tube, primer may be digested by the 3’ to 5’ exonuclease activity of Uni PyroMax polymerase.

94°C

5 min

1 cycle

98°C

10 sec

30 cycle

Annealing*

30 sec

72°C

1 min*

72°C

5 min

1 cycle

*Annealing temperature : Tm - (~ 5 °C) / Tm : 2 X (A+T) + 4 X (G + C)

*Extension time
~ 1 kb : 1 min
> 2 kb : 1.5 ~ 2 /kb