DNA Polymerase >Uni Taq PLUS Polymerase > Uni Taq PLUS (with dNTPs) |
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Uni Taq PLUS (with dNTPs) |
제품의 구성 |
Products |
Size |
Contents |
Cat. No |
Uni Taq PLUS |
250 unit |
10X PCR buffer I |
1 ml |
EP-002A |
10 X PCR buffer II |
1 ml |
500 unit |
10X PCR buffer I |
1 ml X 2 |
EP-002B |
10 X PCR buffer II |
1 ml X 2 |
Uni Taq PLUS
(with dNTPs) |
250 unit |
10X PCR buffer I |
1 ml |
EPD-002A |
10 X PCR buffer II |
1 ml |
2.5 mM dNTPs |
0.8 ml |
500 unit |
10X PCR buffer I |
1 ml X 2 |
EPD-002B |
10 X PCR buffer II |
1 ml X 2 |
2.5 mM dNTPs |
1.6 ml |
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Protocol |
General reaction mixture for PCR (100 ㎕) |
Uni Taq ( 5 U/㎕) |
0.5 ㎕ |
2.5 mM dNTPs |
8 ㎕ |
10 X PCR buffer I, II or mix |
10 ㎕ |
Template DNA |
10 ~ 100 ng |
Primer 1 (10 pmole) |
1 ~ 2 ㎕ |
Primer 2 (10 pmole) |
1 ~ 2 ㎕ |
Sterilized deionized water |
Up to 100 ㎕ |
General reaction cycle profile |
95°C |
3 min |
1 cycle |
94°C |
30 sec |
30 cycle |
Annealing* |
30 sec |
72°C |
~ 2 min* |
72°C |
5 min |
1 cycle |
*Annealing temperature : Tm - (~ 5 °C) / Tm : 2 X (A+T) + 4 X (G + C) |
*Extension time
~ 2 kb : 1 min
> 2 kb : 1 min + (2.5 sec / 100 bases)
If PCR fragments are to be cloned into T/A vectors, the last extension step can be extended to up to 30 min. |
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Experimental data |
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