Uni Taq PLUS

250 unit

10X PCR buffer I

1 ml

EP-002A

10 X PCR buffer II

1 ml

500 unit

10X PCR buffer I

1 ml X 2

EP-002B

10 X PCR buffer II

1 ml X 2

Uni Taq PLUS
(with dNTPs)

250 unit

10X PCR buffer I

1 ml

EPD-002A

10 X PCR buffer II

1 ml

2.5 mM dNTPs

0.8 ml

500 unit

10X PCR buffer I

1 ml X 2

EPD-002B

10 X PCR buffer II

1 ml X 2

2.5 mM dNTPs

1.6 ml

Uni Taq ( 5 U/㎕)

0.5 ㎕

2.5 mM dNTPs

8 ㎕

10 X PCR buffer I, II or mix

10 ㎕

Template DNA

10 ~ 100 ng

Primer 1 (10 pmole)

1 ~ 2 ㎕

Primer 2 (10 pmole)

1 ~ 2 ㎕

Sterilized deionized water

Up to 100 ㎕

95°C

3 min

1 cycle

94°C

30 sec

30 cycle

Annealing*

30 sec

72°C

~ 2 min*

72°C

5 min

1 cycle

*Annealing temperature : Tm - (~ 5 °C) / Tm : 2 X (A+T) + 4 X (G + C)

*Extension time
~ 2 kb : 1 min
> 2 kb : 1 min + (2.5 sec / 100 bases)
If PCR fragments are to be cloned into T/A vectors, the last extension step can be extended to up to 30 min.